From: Bulk milk ELISA and the diagnosis of parasite infections in dairy herds: a review
Term | Explanation |
---|---|
Gold standard | A perfect definitive test that produces no misclassifications |
Sensitivity | Probability of a positive test result given the animal is truly infected |
Specificity | Probability of a negative test result given the animal is truly not infected |
Titre | The highest dilution of the sample at which the test is still positive |
Optical density (OD) | Colour (absorbance) change in a sample resulting from the conversion of substrate and measured using spectrophotometry |
Cut-off OD value | The absorbance above which samples are considered positive |
Receiver operator characteristic | Statistical method used to calculate cut-off OD values |
Sample to positive (SP) ratio or Optical density ratio (ODR) | The ratio of the OD of the sample to the OD of the positive control (SP ratio = ODsample/ODpositive control) |
Percent positivity (PP) | Sample to positive ratio x100 |