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Table 7 ELISA assays for the detection of N. caninum in cattle bulk milk samples

From: Bulk milk ELISA and the diagnosis of parasite infections in dairy herds: a review

ELISA assay

Capture antigen

Sensitivity (95% confidence interval)

Specificity (95% confidence interval)

Reference

ISCOM ELISA (Boehringer Ingelheim Svanova, Uppsala Sweden)

Tachyzoite antigen mixed with iscoms 1

50%

81%

[65]

(21-79%)

(72-89%)

 

IDEXX Neospora antibody test

Whole sonicated tachyzoites

61%

92%

[66]

(49-73%)

(87-98%)

 

LSI ELISA (Lissieu, France)

Whole tachyzoite crude antigen lysate

47%

94%

[66]

(35-60%)

(90-99%)

 

Mastazyme® ELISA (Mast Diagnostics UK)

Whole tachyzoites

61-78%

75-96%

[67]

BioK 192/5, Jemelle, Belgium

Recombinant NcSRS2 protein

95%

96%

[7]

  1. 1 Immunostimulatory complex composed of quillaja saponin, cholesterol and phospholipids.