Figure 2
Comparison of scatterplots of immunophenotyping data from the personal and conventional flow cytometers for leukemic blood. Peripheral blood sample from a dog diagnosed with (CD 21+) large B-cell lymphoma based on clinical findings, lymph node cytology and lymph node immunophenotyping. The dog had a WBC count of 38.7×109/L (ADVIA 2120) with 55% lymphocytes (21.3×109/L). Predominance of (CD21+) lymphocytes (area R5 of the scatterplots). Scatterplots (A) and (C) show the total events as acquired by the personal and the conventional analyzer respectively (2 times more events were acquired by the conventional for this sample). The lymphocyte gate is showed containing 34% of the total events for the Guava and 53% for the conventional analyzer. Scatterplots (B) and (D) illustrate the immunophenotyping results: the Guava gave 64% (CD21+), 10.2% (CD3+), 21.7% double positive and 4.3% double negative. The conventional analyzer gave 71.7% (CD21+), 10.1% (CD3+), 13% double positive and 5.2% double negative events (Dako Summit v4.3 was used to analyze data).