Table 1 Oligonucleotide primers and probes used in the real-time PCR assay for the detection of Brucella spp., B. abortus, and B. melitensis

Brucella spp.

5`GCTCGGTTGCCAATATCAATGC 3`

Forward

Jena Bioscience GmbH, Germany

5`GGGTAAAGCGTCGCCAGAAG 3`

Reverse

AAATCTTCCACCTTGCCCTTGCCATCA 6-FAM/BHQ1

Probe

B. abortus

5`GCGGCTTTTCTATCACGGTATTC 3`

Forward

5`CATGCGCTATGATCTGGTTACG 3`

Reverse

CGCTCATGCTCGCCAGACTTCAATG HEX/BHQ1

Probe

B. melitensis

5`AACAAGCGGCACCCCTAAAA 3`

Forward

5`CATGCGCTATGATCTGGTTACG 3`

Reverse

CAGGAGTGTTTCGGCTCAGAATAATCCACA CY5/BHQ2

Probe