Table 1 Primers used for the identification of bacterial isolates, capsule typing, and the toxA gene
Strains | Target genes | Name | Sequences (5’ → 3’) | Product (bp) | References |
|---|---|---|---|---|---|
P. multocida | kmt1 | Pm-1 | ATCCGCTATTTACCCAGTGG | 457 | [17] |
Pm-2 | GCTGTAAACGAACTCGCCAC | ||||
P. multocida (serotype A) | hyaD-hyaC | APm-1 | GATGCCAAAATCGCAGTCAG | 1048 | [7] |
APm-2 | TGTTGCCATCATTGTCAGTG | ||||
P. multocida (serotype B) | dcbD | BPm-1 | CATTTATCCAAGCTCCACC | 758 | [7] |
BPm-2 | GCCCGAGAGTTTCAATCC | ||||
P. multocida (serotype D) | dcbF | DPm-1 | TTACAAAAGAAAGACTAGGAGCCC | 647 | [7] |
DPm-2 | CATCTACCCACTCAACCATATCAG | ||||
P. multocida (serotype E) | ecbJ | EPm-1 | TCCGCAGAAAATTATTGACTC | 512 | [7] |
EPm-2 | GCTTGCTGCTTGATTTTGTC | ||||
P. multocida (serotype F) | fcbD | FPm-1 | AATCGGAGAACGCAGAAATCAG | 852 | [7] |
FPm-2 | TTCCGCCGTCAATTACTCTG | ||||
P. multocida (toxA gene) | toxA | toxA-1 | CTTAGATGAGCGACAAGG | 864 | [24] |
toxA-2 | GAATGCCACACCTCTATAG | ||||
S. suis | gdh | Ss-1 | GCAGCGTATTCTGTCAAACG | 689 | [20] |
Ss-2 | CCATGGACAGATAAAGATGG | ||||
HPS | 16S rRNA | HPS-1 | TATCGRGAGATGAAAGAC | 1086/1090 | [21] |
HPS-1’ | GTAATGTCTAAGGACTAG | ||||
HPS-2 | CCTCGCGGCTTCGTC | ||||
E. coli | uidA | Ec-1 | ATGAAAGCTGGCTACAGGAAGGCC | 264 | [16] |
Ec-2 | GGTTTATGCAGCAACGAGACGTCA | ||||
Bb | flaB | Bb-1 | TGGCGCCTGCCCTATC | 237 | [19] |
Bb-2 | AGGCTCCCAAGAGAGAAA | ||||
Salmonella | invA | Sal-1 | CAGGATACCTATAGTGCTGC | 580 | [18] |
Sal-2 | CGCACCGTCAAAGGAACCGT |