Table 1 Primer sequences for Teladorsagia circumcincta isotype 1 β–tubulin amplification and sequencing
From: Genetic basis of benzimidazole resistance in Teladorsagia circumcincta in Ireland
Primer Name | Function | Sequence 5′ - 3′ |
|---|---|---|
Tcirc_btubulinF | amplification | CCTCGACTACAATCATGCGTG |
Tcirc_btubulinR | amplification | CGCAACCAATGTGTATTTCG |
Tcirc_btubulin_intF | amplification | GGACAACTTTTCCGTCCAGA |
Tcirc_btubulin_intR | amplification | CAACCCTCTGCCTCTTTACG |
TCSQ1R | sequencing | AGCCAGGACAGAGAACAACG |
TCSQ2F | sequencing | AGTCCAGGAGAGGGGAAAAA |
TCSQ3F | sequencing | AATGCGAAAGTGCTTCACCT |
TCSQ4R | sequencing | AACTGTCCAGGGAATCGAAG |
TCSQ5F | sequencing | TGCTTCCGCACCTTAAAACT |
TCSQ6F | sequencing | CCCACGACTGCTTTGTGTAA |