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Fig. 3 | Irish Veterinary Journal

Fig. 3

From: Application of antibodies to recombinant heat shock protein 70 in immunohistochemical diagnosis of mycobacterium avium subspecies paratuberculosis in tissues of naturally infected cattle

Fig. 3

Immunohistochemical staining of Mycobacterium avium subsp. paratuberculosis in bovine intestinal mucosa using anti-MAP HSP70 antibodies. a A section through the mucosa showing many macrophages containing mycobacteria staining positive with Anti-HSP70 antibodies (DAB substrate-chromagen, and haematoxylin counter staining). b Higher magnification of a. Macrophages (arrow with long tail) and epithelioid cells, stained immunohistochemically using anti-HSP70 antibodies. Note the diffuse character of the staining within the cells. Bar = 17.5μM. c A negative control section in which the Primary antibodies were substituted by pre-immune rabbit serum. No positive staining was observed (DAB substrate-chromagen, and haematoxylin counter staining). Bar = 140μm

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Irish Veterinary Journal

ISSN: 2046-0481