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Table 2 Primers used in PCR for detection of virulence genes in antimicrobial resistant non-typhoidal Salmonella

From: Molecular characterization of antimicrobial resistant non-typhoidal Salmonella from poultry industries in Korea

Primer Broad function (gene function) Sequence (5’ to 3’) Referance
invA Host recognition/invasion (type III secretion system appratus) F-CTGGCGGTGGGTTTTGTTGTCTTCTCTATT Skyberg et al., (2006) [34]
R-AGTTTCTCCCCCTCTTCATGCGTTACCC
orgA Host recognition/invasion (type III secretion system appratus) F-TTTTTGGCAATGCATCAGGGAACA Skyberg et al., (2006) [34]
R-GGCGAAAGCGGGGACGGTATT
prgH Host recognition/invasion (type III secretion system appratus) F-GCCCGAGCAGCCTGAGAAGTTAGAAA Skyberg et al., (2006) [34]
R-TGAAATGAGCGCCCCTTGAGCCAGTC
sopB Host recognition/invasion (type III secretion system appratus) F-CGGACCGGCCAGCAACAAAACAAGAAGAAG Skyberg et al., (2006) [34]
R-TAGTGATGCCCGTTATGCGTGAGTGTATT
tolC Host recognition/invasion (outer membrane channel protein) F-TACCCAGGCGCAAAAAGAGGCTATC Skyberg et al., (2006) [34]
R-CCGCGTTATCCAGGTTGTTGC
sopE Host recognition/invasion (invasion-associated secreted protein) F-CATAGCGCCTTTTCTTCAGG Suez et al., (2013) [35]
R-ATGCCTGCTGATGTTGATTG
sseI Host recognition/invasion (type III secretion system effector protein) F-CGCCATCATCAGTAACCGCC Suez et al., (2013) [35]
R-CTGCTGACCACATCCTCCC
ssek3 Host recognition/invasion (type III secretion system effector protein) F-TATCAATCTCAAATCATGG Suez et al., (2013) [35]
R-CGCGTTTATATCATACGTTTGC
sspH1 Host recognition/invasion (type III secretion system effector protein) F-GGTCACAGGACACGTTCTACG Suez et al., (2013) [35]
R-GCGCTTCTTCGTAATTTTCC
cdtB Host recognition/invasion (toxin-like protein) F-ACAACTGTCGCATCTCGCCCCGTCATT Skyberg et al., (2006) [34]
R-CAATTTGCGTGGGTTCTGTAGGTGCGAGT
hlyE Host recognition/invasion (hemolysis-inducing protein) F-GCGTGATTGAAGGGAAATTG Suez et al., (2013) [35]
R-CGAAAAGCGTCTTCTTACCG
lpfC Host recognition/invasion (fimbrial protein) F-GCCCCGCCTGAAGCCTGTGTTGC Skyberg et al., (2006) [34]
R-AGGTCGCCGCTGTTTGAGGTTGGATA
pefA Host recognition/invasion (fimbial protein) F-TAAGCCACTGCGAAAGATGC Suez et al., (2013) [35]
R-GCGTGAACTCCAAAAACCCG
tcfA Host recognition/invasion (fimbrial protein) F-TCGCTATGTTTGCATGTGGT Suez et al., (2013) [35]
R-TTCAGGAACAGCCTCGAAGT
span Entry into nonphagocytic cells (type III secretion system appratus) F-AAAAGCCGTGGAATCCGTTAGTGAAGT Skyberg et al., (2006) [34]
R-CAGCGCTGGGGATTACCGTTTTG
sipB Entry into nonphagocytic cells (translocation machinery component) F-GGACGCCGCCCGGGAAAAACTCTC Skyberg et al., (2006) [34]
R-ACACTCCCGTCGCCGCCTTCACAA
spiA Survival within macrophage (outer membrane secretory protein) F-CCAGGGGTCGTTAGTGTATTGCGTGAGATG Skyberg et al., (2006) [34]
R-CGCGTAACAAAGAACCCGTAGTGATGGATT
msgA Survival within macrophage (macrophage survival protein) F-GCCAGGCGCACGCGAAATCATCC Skyberg et al., (2006) [34]
R-GCGACCAGCCACATATCAGCCTCTTCAAAC
pagC Survival within macrophage (virulence membrane protein) F-CGCCTTTTCCGTGGGGTATGC Skyberg et al., (2006) [34]
R-GAAGCCGTTTATTTTTGTAGAGGAGATGTT
sodC Survival within macrophage (periplasmic Cu/Zn superoxide dismutase) F-ATGACACCACAGGCAAAACG Suez et al., (2013) [35]
R-AGATGAACGATGCCCTGTCC
gatC Growth within host (PTS galactitol transporter subunit IIC) F-ATTGGTATCGGCTTCGTGGG Suez et al., (2013) [35]
R-ATCCCCAGCCAGTATGAACC
spvB Growth within host (ADP-ribosylating toxin) F-CTATCAGCCCCGCACGGAGAGCAGTTTTTA Skyberg et al., (2006) [34].
R-GGAGGAGGCGGTGGCGGTGGCATCATA
sitC Iron acquisition (permease) F-CAGTATATGCTCAACGCGATGTGGGTCTCC Skyberg et al., (2006) [34]
R-CGGGGCGAAAATAAAGGCTGTGATGAAC
iron Iron acquisition (sidrophore) F-ACTGGCACGGCTCGCTGTCGCTCTAT Skyberg et al., (2006) [34]
R-CGCTTTACCGCCGTTCTGCCACTGC
sifA Filamentous structure formation (secreted effector protein) F-TTTGCCGAACGCGCCCCCACACG Skyberg et al., (2006) [34]
R-GTTGCCTTTTCTTGCGCTTTCCACCCATCT
STM 2759 Putative dipeptice/oligopetide/nikel ABC-type transport systems F-ACCATTTTCACCTGGGCTCC Suez et al., (2013) [35]
R-CGTTCAGGTTTTGTCGCTGG