Table 1 Primers used in this study
Target | Sequence (5' → 3') | Size (bp) | References |
|---|---|---|---|
Identification | |||
malB | F: GACCTCGGTTTAGTTCACAGA | 585 | [17] |
R: CACACGCTGACGCTGACCA | |||
β-lactamases | |||
CTX-M group I | F: GACGATGTCACTGGCTGAGC | 499 | [20] |
R: AGCCGCCGACGCTAATACA | |||
CTX-M group II | F: GCGACCTGGTTAACTACAATCC | 351 | [20] |
R: CGGTAGTATTGCCCTTAAGCC | |||
CTX-M group III | F: CGCTTTGCCATGTGCAGCACC | 307 | [20] |
R: GCTCAGTACGATCGAGCC | |||
CTX-M group IV | F: GCTGGAGAAAAGCAGCGGAG | 474 | [20] |
R: GTAAGCTGACGCAACGTCTG | |||
TEM | F: CATTTCCGTGTCGCCCTTATTC | 800 | [21] |
R: CGTTCATCCATAGTTGCCTGAC | |||
SHV | F: CACTCAAGGATGTATTGTG | 885 | [22] |
R: TTAGCGTTGCCAGTGCTCG | |||
OXA | F: TTCAAGCCAAAGGCACGATAG | 702 | [22] |
R: TCCGAGTTGACTGCCGGGTTG | |||
Aminoglycoside-modifying enzymes | |||
aac(6′)-Ib | F: TGACCTTGCGATGCTCTATG | 509 | [23] |
R: TTAGGCATCACTGCGTGTTC | |||
aac(3)-II | F: TGAAACGCTGACGGAGCCTC | 369 | [24] |
R: GTCGAACAGGTAGCACTGAG | |||
ant(2″)-I | F: GGGCGCGTCATGGAGGAGTT | 740 | [24] |
R: TATCGCGACCTGAAAGCGGC | |||
aph(3″)-Ib | F: CTTGGTGATAACGGCAATTCC | 548 | [25] |
R: CCAATCGCAGATAGAAGGCAA | |||
ant(4′)-IIa | F: ATCGTCTGCGAGAAGCGTAT | 839 | [25] |
R: TAAAACGCCTATCCGTCACC | |||
Virulence factors | |||
eaeA | F: TCAATGCAGTTCCGTTATCAGTT | 482 | [26] |
R: GTAAAGTCCGTTACCCCAACCTG | |||
escV | F: ATTCTGGCTCTCTTCTTCTTTATGGCTG | 544 | [26] |
R: CGTCCCCTTTTACAAACTTCATCGC | |||
stx1 | F: CGATGTTACGGTTTGTTACTGTGACAGC | 244 | [26] |
R: AATGCCACGCTTCCCAGAATTG | |||
fimH | F: AACAGCGATGATTTCCAGTTTGTGTG | 465 | [27] |
R: ATTGCGTACCAGCATTAGCAATGTCC | |||
iucC | F: AAACCTGGCTTACGCAACTGT | 269 | [27] |
R: ACCCGTCTGCAAATCATGGAT | |||
iutA | F: GGCTGGACATCATGGGAACTGG | 300 | [28] |
R: CGTCGGGAACGGGTAGAATCG | |||
fyuA | F: TGATTAACCCCGCGACGGGAA | 880 | [28] |
R: CGCAGTAGGCACGATGTTGTA | |||
Integron | |||
class 1 integrase | F: GCCACTGCGCCGTTACCACC | 898 | [29] |
R: GGCCGAGCAGATCCTGCACG | |||
class 2 integrase | F: CACGGATATGCGACAAAAAGGT | 789 | [30] |
R: GTAGCAAACGAGTGACGAAATG | |||
Phylogenetic group | |||
chuA | F: GACGAACCAACGGTCAGGAT | 279 | [31] |
R: TGCCGCCAGTACCAAAGACA | |||
yjaA | F: TGAAGTGTCAGGAGACGCTG | 211 | [31] |
R: ATGGAGAATGCGTTCCTCAAC | |||
TspE4.C2 | F: GAGTAATGTCGGGGCATTCA | 152 | [31] |
R: CGCGCCAACAAAGTATTACG | |||