Skip to main content

Table 7 PCR assay reaction of the current isolates

From: Serotyping, antibiogram, and detection of bacterial pathogens associated with bovine respiratory disease in selected areas of Ethiopia

Isolates

PCR reaction

Final extension

Initial denaturation

Denaturation

(35 cycles)

Annealing

Extension

M. haemolytica

95 °C; 3 min

95 °C; 1 min

48 °C; 1 min

72 °C; 1 min

72 °C; 5 min

P. multocida

95 °C; 5 min

95 °C; 1 min

55 °C; 1 min

72 °C; 1.5 min

72 °C; 7 min.

B. trehalosi

95 °C; 5 min

95 °C; 30 s

55 °C; 30 s

72 °C; 40 s

72 °C; 5 min.

Back to article page

Irish Veterinary Journal

ISSN: 2046-0481