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Fig. 1 | Irish Veterinary Journal

Fig. 1

From: Application of antibodies to recombinant heat shock protein 70 in immunohistochemical diagnosis of mycobacterium avium subspecies paratuberculosis in tissues of naturally infected cattle

Fig. 1

An agarose gel electrophoresis of HSP70 gene amplified product and restriction analysis of PET-SUMO + HSP70 recombinant plasmid. a The open reading frame of HSP70 gene, 1872bp in length was amplified. The PCR product in a was later cloned in PET-SUMO vector (Invitrogen). b The restriction patterns of HSP70 cloned in frame with PET-SUMO vector. From left, lane 1 is the DNA ladder, lanes 2 is a recombinant with the gene in a 3’-5’direction (wrong orientation) showing two bands 6720bpand 910bp, lane 3 and 4 are non-recombinant plasmids without the insert(≈5643bp), lane 5 is the plasmid with an insert in the correct 5’-3’ orientationshowing two fragments 6445bp and 1185bp. The DNA ladder used is the NEB 1kb ladder. The bar chart to the left of A shows the sizes of the DNA fragments on the ladder, which in turn shows the size of the amplified products in gel a and b

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Irish Veterinary Journal

ISSN: 2046-0481