Fig. 5

Partial phylogenetic tree of Michigan, USA, M. bovis isolates, illustrating genotypic variation from an Alcona County beef herd (Herd 77) first diagnosed M. bovis positive in January 2020. Over the course of the next eleven months, eight distinct genotypes were identified via whole genome sequencing, including two from three lymph node pools of a single animal (arrows). Genotypes identified in cattle from this herd and two infected trace out herds (Herd 78 from Allegan County and Feedlot F-6 from Saginaw County) were from three to six single nucleotide polymorphisms (SNPs, i.e., point mutations) from sharing a common ancestor with isolates collected from free-ranging white-tailed deer in Alcona County in 2015 and 2017