Table 1 PRC conditions for each protocol
From: Sensitivity of two methods to detect Mycoplasma agalactiae in goat milk
Reference | Primers | PCR conditions |
|---|---|---|
Tola et al. (1996) [8] | 5′-AAAGGTGCTTGAGAAATGGC-3′ | 94 °C, 5 min.; (94 °C, 1 min. 64 °C, 1 min.; 72 °C,1 min) x 35 cycles; 72 °C, 10 min. |
5′-GTTGCAGAAGAAAGTCCAATCA-3′ | ||
Marenda et al. (2005) [5] | 5′-CATTGAACCTCTTATGTCATTTACTTTG-3′ | 94 °C, 5 min.; (94 °C, 1 min.; 58 °C, 1 min.; 72 °C, 1 min.) x 30 cycles; 72 °C, 10 min. |
5′-CTATGTCATCAGCTTTTGGGTGA-3′ | ||
De la Fe et al. (2012) [6] | 5′-GCAGCTTGTTTAGTGTCAAAG-3′ | 94 °C, 2 min.; (94 °C, 30 sec.; 49 °C, 30 sec,; 72 °C, 30 sec.) x 30 cycles; 72 °C, 5 min. |
5′-CCTAAAGCAACCTTTATAACTG-3′ |