Table 2 PCR results obtained according to the DNA extraction method and the Mycoplasma agalactiae concentration used
From: Sensitivity of two methods to detect Mycoplasma agalactiae in goat milk
M. agalactiae concentration (CFU/ml) | Method of extraction | |||||
|---|---|---|---|---|---|---|
Tola et al. (1997) [8] | Commercial kitc | |||||
Negative | Positive | Se (%), 95 % CI | Negative | Positive | Se (%), 95 % CI | |
101 | 30 | 0 | 0 | 30 | 0 | 0 |
102 | 30 | 0 | 0 | 29 | 1 | 3.3, (−3.1 %, 9.8 %) |
103 | 30 | 0 | 0 | 24 | 6 | 20, (5.7 %, 34.3 %) |
104 | 28 | 2 | 6,7, (−2.3 %, 15.6 %) | 22 | 8 | 26.7, (10.8 %, 42.5 %) |
105 | 15 | 15 | 50, (32.1 %, 67.9 %) | 8 | 22 | 73.3, (57.5 %, 89.2 %) |
106 | 2 | 28 | 93,3, (84.4 %, 102.3 %) | 0 | 30 | 100 |
107 | 0 | 24 | 100 | 0 | 24 | 100 |
Total | 135 | 69 | 33.8,a (37.8 %, 51.4 %) | 113 | 91 | 44.6,b (27.3 %, 40.3 %) |