Fig. 1From: Application of antibodies to recombinant heat shock protein 70 in immunohistochemical diagnosis of mycobacterium avium subspecies paratuberculosis in tissues of naturally infected cattleAn agarose gel electrophoresis of HSP70 gene amplified product and restriction analysis of PET-SUMO + HSP70 recombinant plasmid. a The open reading frame of HSP70 gene, 1872bp in length was amplified. The PCR product in a was later cloned in PET-SUMO vector (Invitrogen). b The restriction patterns of HSP70 cloned in frame with PET-SUMO vector. From left, lane 1 is the DNA ladder, lanes 2 is a recombinant with the gene in a 3’-5’direction (wrong orientation) showing two bands 6720bpand 910bp, lane 3 and 4 are non-recombinant plasmids without the insert(≈5643bp), lane 5 is the plasmid with an insert in the correct 5’-3’ orientationshowing two fragments 6445bp and 1185bp. The DNA ladder used is the NEB 1kb ladder. The bar chart to the left of A shows the sizes of the DNA fragments on the ladder, which in turn shows the size of the amplified products in gel a and b Back to article page