Table 2 Primer sequences used to amplify fragments of the rRNA genes and internal transcribed spacers
Locus (size) | Primer | Sequence | Ref |
|---|---|---|---|
18S (700 bp) | External | Fw: 5′ AAAGATTAAGCCATGCA 3′ Rev.: 5’GCAGGTTCACCTACAGAT 3’ | [22] |
Nested | Fw: 5’ CGGCTCATTAGAGCAGATGTC 3′ Rev.: 5′ TCCTCTTTTATTATTCCATGATCG 3’ | This study | |
ITS2 (548 bp) | External | Fw: 5’ TTTGAACGCATAGCGTCGT 3′ Rev.: 5′ TTAGTTTCTTTTCCTCCGCT 3’ | This study [23] |
Hemi-nested | Fw: 5’ ACGTCTGGTTCAGGGTTGTT 3′ Rev.: 5′ TTAGTTTCTTTTCCTCCGCT 3’ | [23] | |
Connecting fragment (1953 bp) | External | Fw: 5’ GCCTTTGGCGTTAATCACTG 3′ Rev.: 5′ TCACGATCATCAATCCATCG 3’ | This study |
Nested | Fw: 5’ AGAACAAGCGTTTGCTTGAA3′ Rev.: 5′ GTATGACCAACAGCCGAACA3’ | This study |