Table 2 Primers used for genetic resistance profiling and virulence gene detection by PCR
Gene | Primers | Product size (bp) |
|---|---|---|
tet(M) | 5′-ACAGAAAGCTTATTATATAAC-3′ 5′-TGGCGTGTCTATGATGTTCAC-3′ | 171 |
erm(B) | 5′-GAAAAGGTACTCAACCAAATA-3′ 5′-AGTAACGGTACTTAAATTGTTTAC-3 | 639 |
aac(6′)-Ie-aph(2″)-Ia | 5′-CCAAGAGCAATAAGGGCATA-3′ 5′-CACTATCATAACCACTACCG-3′ | 369 |
pbp5 | 5′ CATGCGCAATTAATCGG 3′ 5′ CATAGCCTGTCGCAAAAC 3′ | 444 |
cylA | 5′-TAGCGAGTTATATCGTTCACTGTA-3′ 5′-CTCACCTCTTTGTATTTAAGCATG-3́ | 1282 |
gelE | 5′-ACCCCGTATCATTGGTTT-3′ 5′-ACGCATTGCTTTTCCATC-3´ | 419 |
esp | 5´-TTGCTAATGCTAGTCCACGACC −3′ 5′-GCGTCAACACTTGCATTGCCGAA-3´ | 993 |
agg | 5´-AAGAAAAAGAAGTAGACCAAC-3′ 5′-AAACGGCAAGACAAGTAAATA-3´ | 1553 |