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Table 2 Primers used for genetic resistance profiling and virulence gene detection by PCR

From: Resistance and virulence distribution in enterococci isolated from broilers reared in two farming systems

Gene Primers Product size (bp)
tet(M) 5′-ACAGAAAGCTTATTATATAAC-3′
5′-TGGCGTGTCTATGATGTTCAC-3′
171
erm(B) 5′-GAAAAGGTACTCAACCAAATA-3′
5′-AGTAACGGTACTTAAATTGTTTAC-3
639
aac(6′)-Ie-aph(2″)-Ia 5′-CCAAGAGCAATAAGGGCATA-3′
5′-CACTATCATAACCACTACCG-3′
369
pbp5 5′ CATGCGCAATTAATCGG 3′
5′ CATAGCCTGTCGCAAAAC 3′
444
cylA 5′-TAGCGAGTTATATCGTTCACTGTA-3′
5′-CTCACCTCTTTGTATTTAAGCATG-3́
1282
gelE 5′-ACCCCGTATCATTGGTTT-3′
5′-ACGCATTGCTTTTCCATC-3´
419
esp 5´-TTGCTAATGCTAGTCCACGACC −3′
5′-GCGTCAACACTTGCATTGCCGAA-3´
993
agg 5´-AAGAAAAAGAAGTAGACCAAC-3′
5′-AAACGGCAAGACAAGTAAATA-3´
1553
  1. Abbreviations: tet(M) tetracycline resistance gene, erm(B) macrolide resistance gene, aac (6′)-Ie-aph (2″)-Ia aminoglycoside resistance gene, pbp5 penicillin resistance gene, cylA cytolisin A gene, gelE gelatinase E gene, esp enterococcal surface protein gene, agg aggregation substance gene, bp base pairs