Serotyping, antibiogram, and detection of bacterial pathogens associated with bovine respiratory disease in selected areas of Ethiopia

Table 6 Isolates and target gene sequences (5′ to 3′) used in PCR assay

Isolates	Gene	Primers	Sequence (5′ to 3′)	Size (bp)	Reference
M. haemolytica	PHSSA	PHSSA (F)	TTC ACA TCT TCA TCC TC	325	[25]
	PHSSA	PHSSA (R)	TTT TCA TCC TCT TCG TC	325
	Rpt2	Rpt2 (F)	GTT TGT AAG ATA TCC CAT TT	1022
	Rpt2	Rpt2 (R)	CGT TTT CCA CTT GCG TGA	1022
P. multocida	KMT1	KMT1T7 (F)	ATC CGC TAT TTA CCC AGT GG	460	[15, 26]
P. multocida	KMT1	KMT1SP6(R)	GCT GTA AAC GAA CTC GCC AC	460	[15, 26]
Serogroup A	hyaD-hyaC	capA (F)	TGC CAA AAT CGC AGT CAG	1044	[16]
Serogroup A	hyaD-hyaC	capA (R)	TTG CCA TCA TTG TCA GTG	1044	[16]
Serogroup B	bcbD	capB (F)	CAT TTA TCC AAG CTC CAC C	760	[16]
Serogroup B	bcbD	capB (R)	GCC CGA GAG TTT CAA TCC	760	[16]
Serogroup D	dcbF	capD (F)	TTA CAA AAG AAA GAC TAG GAG CCC	657	[16]
Serogroup D	dcbF	capD (R)	CAT CTA CCC ACT CAA CCA TAT CAG	657	[16]
Serogroup E	ecbJ	capE (F)	TCC GCA GAA AAT TAT TGA CTC	511	[16]
Serogroup E	ecbJ	capE (R)	GCT TGC TGC TTG ATT TTG TC	511	[16]
B. trehalosi	sodA	BtsodA (F)	GCC TGC GGA CAA ACG TGT TG	144	[31]
B. trehalosi	sodA	BtsodA (R)	TTT CAA CAG AAC CAA AAT CAC GAA TG	144	[31]

ISSN: 2046-0481