Fig. 2
From: First report of Besnoitia bennetti in Irish donkeys: an emerging parasitic disease in Europe
Case 1, donkey, sarcoid and Besnoitia cysts: a numerous white pinpoint cysts present on the conjunctiva, b Initial presentation of raised broad based ulcerated dermal nodule on the muzzle, c Muzzle four weeks after surgery and trimethoprim sulfadiazine treatment, pinpoint white cysts visible (arrows), d Photomicrograph illustrating a tissue cyst with the characteristic three layers: a hyaline outer layer (Giemsa positive (e) consisting of collagen (Masson trichrome positive (f)), a middle layer composed of host cell cytoplasm with multiple nuclei and a thin inner membrane enclosing a parasitophorous vacuole with the bradyzoities, haematoxylin eosin