Spinal neurenteric cyst in a dog
© Ferrand et al.; licensee BioMed Central. 2015
Received: 14 October 2014
Accepted: 11 May 2015
Published: 22 May 2015
A 2-year-old female crossbreed dog was presented with progressive ataxia and paraparesis. A T3-L3 spinal lesion was determined by neurological examination. Magnetic resonance imaging (MRI) revealed an ovoid-shaped, well-circumscribed mass affecting the spinal cord at the level of the T9 vertebra. A left hemilaminectomy and a durotomy at the level of T9 allowed discovery of an ovoid deformation of the meninges with a cystic appearance.
En bloc removal was performed and appeared to be complete. Pathological analysis showed a voluminous cystic lesion lined by a heterogeneous epithelium. Three types of epithelium were present: a pseudostratified columnar epithelium, a stratified squamous epithelium and a transitional epithelium. Mucus production, the morphology of some cells with microvilli at the apical pole and immunohistochemical assays were highly in favor of an endodermal origin of the cyst. The age of the dog, anamnesis, MRI study and histological findings were consistent with an intradural neurenteric cyst as described in humans. Total surgical removal led to a progressive clinical improvement with no recurrence at 18 months. We report an unusual intradural extramedullary cyst, called a neurenteric cyst, in a 2-year-old female crossbreed dog. This type of cyst is well-known in humans but has never been described in dogs. We propose that neurenteric cysts should be included in the differential diagnoses for tumor-like or cystic intradural lesions in the young dog. Prognosis for this type of cyst seems to be good, as total surgical removal led to a progressive clinical improvement with no recurrence at 18 months.
Thoraco-lumbar intradural extra-axial myelopathies are uncommon in dogs, especially in young dogs. Pseudocysts and embryonic tumors represent the most common lesions, often causing progressive onset of clinical signs such as paraspinal hyperesthesia, ataxia, paresis, and plegia [1, 2]. True cystic structures are rare. We describe an unusual intradural extramedullary cyst in a 2-year-old female crossbreed dog. Due to its similarity to human findings, a neurenteric cyst is suspected.
Intradural extra-axial “cystic or tumor-like lesions” of the thoraco-lumbar spinal cord are uncommon in young dogs. Histopathological features of epidermoid or dermoid cysts, nephroblastomas or Tarlov cysts are well described [3, 4]. In the present case, the cyst was lined by different types of epithelia that suggested a respiratory, digestive, cutaneous or urinary origin. Structures similar to cilia or a brush border at the apical pole of cuboidal cells and mucus secretion suggested a respiratory or digestive epithelium. The stratified squamous epithelium was similar to a cutaneous epithelium but with no sign of keratinization, in contrast to an epidermoid cyst. Moreover the endodermal origin was supported by the presence of mucus and microvilli at the apical pole of some cells and immunhistochemistry. In humans, a neurenteric cyst, also called an enterogenous cyst or endodermic cyst, is a congenital lesion characterized by a mucus-secreting epithelium mimicking the epithelium of the gastrointestinal tract . Neurenteric cysts result from inappropriate partitioning of the embryonic notochordal plate and presumptive endoderm . A failure during embryogenic development could be responsible for the persistence of an abnormal communication between the endoderm and neuroectoderm at 3 weeks of development in the human embryo . Several theories have been proposed to explain the occurrence of neurenteric cysts . For example Mac Donald et al.  proposed different mechanisms in which neurenteric cysts may develop: a primary adhesion of endoderm anterior to the notochord, incomplete excalation of the notochord, persistence of the neurenteric canal or formation of an accessory neurenteric canal with a split notochord, and displacement of endodermal cells. Bentley and Smith  postulated that the splitting of the notochord is the primary event in this pathology. The subsequent deficiency in the overlying neural plate could allow for an endodermal diverticulum to herniate through the spinal column and make contact with the surface ectoderm. In such a situation, the persistence of the neurenteric connection may be transient or permanent, partial or complete. In the canine fetus, closure of the neural tube occurs at about 20 days, so that we can expect that the neurenteric cyst formation occurs at this period of development in dogs .
A heterotopic epithelium reminiscent of gastrointestinal and respiratory tissue lead to a compressive cystic lesion in the pediatric and adult spine. Histopathological analysis of neurenteric tissue reveals a highly characteristic structure of columnar or cuboidal epithelium with or without cilia and mucus globules, as in the present case. Wilkins and Odum described 3 types of neurenteric cysts : type A cysts contain either columnar or cuboidal cells, with ciliated and nonciliated components atop a basal membrane composed of type IV collagen. Type B cysts include all of the features of type A as well as additional tissue that may include bone, cartilage, lymphatic tissue, fat, or glandular components. Type C cysts are identified by type A features in association with ependymal or glial tissue. We think we can classify the neurenteric cyst in the present case as a type A. Spinal neurenteric cysts account for 0.7-1.3 % of spinal axis “tumors” in humans . Neurenteric cysts are usually intradural and extramedullary lesions , as in the case reported here. According to the literature, the most common location is the cervicodorsal region, ventral to the spinal cord [13, 14]. In the thoracolumbar region, the neurenteric cyst appears to be situated dorsal to the spinal cord . In our case, the cyst was in a dorso-lateral position in relation to the spinal cord. Human patients are typically symptomatic in the second and third decades of life, with a male predominance (sex ratio = 2) , but the reported age of presentation varies from 5 weeks to 52 years . In this case, the dog was also a young adult. Myelopathic and/or radicular signs are size and location-dependent . Pain is the main presenting clinical sign, not neurological deficits, which reflects the slow growth of the cyst . In our case, massive spinal cord compression without dramatic neurological deficits supports the hypothesis of a slow-growing lesion. MRI appears to be the best modality for identifying the complex anatomy of spinal cysts [13, 15]. The cyst appears iso-intense on T1-weighted sequences and hyper-intense on T2-weighted sequences with no contrast enhancement. Edema surrounding the spine is generally not observed [15, 16]. These MRI features are considered typical of a neurenteric cyst, but variations can occur. In our case, the cyst presented the characteristic MRI pattern of a neurenteric cyst. Radiology and computed tomography can also be useful tools for the evaluation of osseous malformations associated with this lesion . Indeed in a case series of 23 human patients with neurenteric cyst, 87 % showed vertebral anomalies at the same level as the cyst on radiographic evaluation .
In humans, immunohistochemistry is key to confirming the endodermic origin of the lesion. Cystic epithelial cells typically stain negatively for gliofibrillar acid protein (GFAP), neuronal specific enolase, vimentine, S100 antibodies, and positively for cytokeratin epithelial membrane antigen and carcinoembryonic antigen (CEA) [6, 12]. Positive CEA staining supports a theory of shared lineage between the cystic wall and the intestinal mucosa. In the case of intramedullary lesions, astrocytes within the cyst wall may stain positively for GFAP in comparison to the typical negative staining pattern of extramedullary cysts .
Total surgical excision led to a good, although progressive, neurological recovery in our patient. Surgical removal is the treatment of choice in humans , resulting in minimal morbidity . Total removal seems to be curative, but recurrence rates as high as 37 % have been reported after incomplete resection due to factors such as cyst adhesion to surrounding structures and unclear dissection planes .
We report an unusual intradural extramedullary cyst in a 2-year-old female crossbreed dog. The MRI study and histological findings are consistent with an intradural neurenteric cyst, as described in humans. The neurenteric cyst should be included in the differential diagnoses for tumor-like or cystic intradural lesions in the young dog. Prognosis for this type of cyst seems to be good, as total surgical removal led to progressive clinical improvement with no recurrence at 18 months.
- Dickinson PJ, Sturges BK, Berry WL, Vernau KM, Koblik PD, Lecouteur RA. Extradural spinal synovial cysts in nine dogs. J Small Anim Pract. 2001;42:502–9.View ArticlePubMedGoogle Scholar
- Skeen TM, Olby NJ, Munana KR, Sharp NJ. Spinal arachnoid cysts in 17 dogs. J Am Anim Hosp Assoc. 2003;39:271–82.View ArticlePubMedGoogle Scholar
- DeDecker S, Davies E, Wilson H, Pelligand L, Rayner EL, Shihab N, et al. Surgical treatment of an intracranial epidermoid cyst in a dog. Vet Surg. 2012;41:766–71.View ArticleGoogle Scholar
- Liebel FX, Platt S, Matiasek K, Houlton J, Garosi L. Diagnosis and management of perineurial (Tarlov) cysts in two dogs. Vet Rec. 2013;172:504.View ArticlePubMedGoogle Scholar
- Harris CP, Dias MS, Brockmeyer DL, Townsend JJ, Willis BK, Apfelbaum RI. Neurenteric cysts of the posterior fossa: recognition, management, and embryogenesis. Neurosurgery. 1991;29:893–8.View ArticlePubMedGoogle Scholar
- Ghannane H, Laghmari M, Aniba K, Lmejjati M, Ait BS. Craniocervical intradural neurenteric cyst. Pan Arab J Neurosurg. 2011;15:64–7.Google Scholar
- Tubbs RS, Salter EG, Oakes WJ. Neurenteric cyst: case report and a review of the potential dysembryology. Clin Anat. 2006;19:669–72.View ArticlePubMedGoogle Scholar
- MacDonald RL, Schwartz ML, Lewis AJ. Neurenteric cyst located dorsal to the cervical spine: Case report. Neurosurgery. 1991;28:587–8.View ArticleGoogle Scholar
- Bentley JL, Smith JR. Developmental posterior enteric remnants and spinal malformations: The split notochord syndrome. Arch Dis Child. 1960;35:76–86.View ArticlePubMed CentralPubMedGoogle Scholar
- Thomson C, Hahn C. Neuroembryology. In: Thomson C, Hahn C, editors. Veterinary neuroanatomy - A clinical approach. Edinburgh: Saunders Elsevier; 2012. p. 11–6.Google Scholar
- Wiklens RH, Odom GL. Spinal intradural cysts. In: Vinkin PJ, Bruyn GW, editors. Tumors of the spine and spinal cord, Part II. Handbook of clinical neurology, vol. 20. Amsterdam: North Holland; 1976. p. 55–102.Google Scholar
- Savage JJ, Casey JN, McNeill IT, Sherman JH. Neurenteric cysts of the spine. J Craniovertebr Junction Spine. 2010;1:58–63.View ArticlePubMed CentralPubMedGoogle Scholar
- Khadim MT, Asif M, Ali A. Intraspinal neurenteric cyst — a rare entity. J Pak Med Assoc. 2011;61:1143–5.PubMedGoogle Scholar
- Rauzzino MJ, Tubbs RS, Alexander E, Grabb PA, Oakes WJ. Spinal neurenteric cysts and their relation to more common aspects of occult spinal dysraphism. Neurosurg Focus. 2001;10:1–13.View ArticleGoogle Scholar
- Evans A, Stoodley N, Halpin S. Magnetic resonance imaging of intraspinal cystic lesions: A pictorial review. Curr Probl Diagn Radiol. 2002;31:79–94.View ArticlePubMedGoogle Scholar
- Pierot L, Dormont D, Queslati S, Cornu P, Rivierez M, Bories J. Gadolinium-DTPA enhanced MR imaging of intradural neurenteric cyst. J Comput Assist Tomogr. 1988;12:762–4.View ArticlePubMedGoogle Scholar
- Garg N, Sampath S, Yasha TC, Chandramouli BA, Devi BI, Kovoor JM. Is total excision of spinal neurenteric cysts possible? Br J Neurosurg. 2008;22:241–51.View ArticlePubMedGoogle Scholar
- Lippman CR, Arginteanu M, Purohit D, Naidich TP, Camins MB. Intramedullary neurenteric cysts of the spine. Case report and review of the literature. J Neurosurg. 2001;94:305–9.PubMedGoogle Scholar
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